{"V1":"1"}
{"V1":"We retrieved PATHNET data, a prior reconstruction of the"}
{"V1":"H. salinarum metabolic network based on Kyoto Encyclopedia"}
{"V1":"of Genes and Genomes (KEGG) defined pathways,58 from the"}
{"V1":"Halolex database. We used this as the starting point for"}
{"V1":"further reconstruction using literature review and bioinfor-"}
{"V1":"matic means. The evidences used for the inclusion of reactions/"}
{"V1":"enzymes to the network include enzymatic assays, labeling"}
{"V1":"studies, and nutrient uptake reports (for transporters)."}
{"V1":"Reactions described by such references were included with or"}
{"V1":"without genetic evidence. Similarly, pathway gaps and reac-"}
{"V1":"tions devoid of literature or genetic indicators but required by"}
{"V1":"the network to produce a compound essential for growth"}
{"V1":"were also added. In cases where detailed information on"}
{"V1":"H. salinarum R-1 were unavailable, data from various strains"}
{"V1":" of Halobacterium salinarum were used. Likewise, reaction"}
{"V1":"reversibilities were also taken from literature sources whenever"}
{"V1":"available. Particularly helpful in this regard was the BRENDA"}
{"V1":"database,59 which, among other things, stores reversibility"}
{"V1":"information from various organisms. "}